Demonstration of the existence of mRNAs encoding N1/ cif and N2/ cit sodium/nucleoside cotransporters in rat brain
Identifieur interne : 003E62 ( Main/Exploration ); précédent : 003E61; suivant : 003E63Demonstration of the existence of mRNAs encoding N1/ cif and N2/ cit sodium/nucleoside cotransporters in rat brain
Auteurs : Christopher M. Anderson [Canada] ; Wei Xiong [Canada] ; James D. Young [Canada] ; Carol E. Cass [Canada] ; Fiona E. Parkinson [Canada]Source :
- Molecular Brain Research [ 0169-328X ] ; 1996.
English descriptors
- KwdEn :
- Adenosine, Adenosine transport, Animals, Biological Transport (physiology), Brain (metabolism), Carrier Proteins (genetics), Male, Membrane Transport Proteins, Molecular Sequence Data, Na+/nucleoside cotransport, Nucleoside transport, Purine Nucleosides (metabolism), RNA, Messenger (metabolism), RT-PCR, Rat brain, Rats, Rats, Sprague-Dawley, Sodium (metabolism).
- MESH :
- chemical , genetics : Carrier Proteins.
- metabolism : Brain, Purine Nucleosides, RNA, Messenger, Sodium.
- physiology : Biological Transport.
- Animals, Male, Membrane Transport Proteins, Molecular Sequence Data, Rats, Rats, Sprague-Dawley.
Abstract
Nucleoside transport may be involved in the regulation of extracellular levels of adenosine, an inhibitory neuromodulator in the central nervous system. Previous reports have provided functional evidence for Na+-dependent nucleoside transport in rat brain. We isolated total RNA from various regions of rat brain and tested for the presence of mRNA for two recently cloned Na+/nucleoside cotransporters using reverse transcriptase PCR (RT-PCR). Messenger RNA for a pyrimidine-selective Na+/nucleoside cotransporter mRNA (rCNTl) was detected in samples from each brain region tested by RT-PCR amplification of a 309-bp DNA product. Southern blot and sequence analysis confirmed that this product was derived from rCNTl mRNA. A purine-selective Na+/nucleoside cotransporter mRNA (rCNT2, also termed SPNT) was detected throughout brain by amplifying a 235-bp DNA product, the sequence of which was identical to that published. These experiments demonstrate the presence of both rCNT1 and rCNT2 mRNA in rat brain.
Url:
DOI: 10.1016/S0169-328X(96)00244-6
Affiliations:
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Young</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Physiology, Faculty of Medicine, University of Alberta, 755 Medical Sciences Building, Edmonton, Alta. T6G 2H7</wicri:regionArea><wicri:noRegion>Alta. T6G 2H7</wicri:noRegion></affiliation></author><author><name sortKey="Cass, Carol E" sort="Cass, Carol E" uniqKey="Cass C" first="Carol E." last="Cass">Carol E. Cass</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Departments of Biochemistry and Oncology, University of Alberta, Cross Cancer Institute, 11560 University Avenue, Edmonton, Alta. T6G 1Z2</wicri:regionArea><wicri:noRegion>Alta. T6G 1Z2</wicri:noRegion></affiliation></author><author><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E." last="Parkinson">Fiona E. Parkinson</name><affiliation wicri:level="4"><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, 770 Bannatyne Avenue, Winnipeg</wicri:regionArea><orgName type="university">Université du Manitoba</orgName><placeName><settlement type="city">Winnipeg</settlement><region type="state">Manitoba</region></placeName></affiliation><affiliation></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Canada</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Brain Research</title><title level="j" type="abbrev">BRESM</title><idno type="ISSN">0169-328X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">42</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="358">358</biblScope><biblScope unit="page" to="361">361</biblScope></imprint><idno type="ISSN">0169-328X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0169-328X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine</term><term>Adenosine transport</term><term>Animals</term><term>Biological Transport (physiology)</term><term>Brain (metabolism)</term><term>Carrier Proteins (genetics)</term><term>Male</term><term>Membrane Transport Proteins</term><term>Molecular Sequence Data</term><term>Na+/nucleoside cotransport</term><term>Nucleoside transport</term><term>Purine Nucleosides (metabolism)</term><term>RNA, Messenger (metabolism)</term><term>RT-PCR</term><term>Rat brain</term><term>Rats</term><term>Rats, Sprague-Dawley</term><term>Sodium (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Carrier Proteins</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Brain</term><term>Purine Nucleosides</term><term>RNA, Messenger</term><term>Sodium</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Biological Transport</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Male</term><term>Membrane Transport Proteins</term><term>Molecular Sequence Data</term><term>Rats</term><term>Rats, Sprague-Dawley</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Nucleoside transport may be involved in the regulation of extracellular levels of adenosine, an inhibitory neuromodulator in the central nervous system. Previous reports have provided functional evidence for Na+-dependent nucleoside transport in rat brain. We isolated total RNA from various regions of rat brain and tested for the presence of mRNA for two recently cloned Na+/nucleoside cotransporters using reverse transcriptase PCR (RT-PCR). Messenger RNA for a pyrimidine-selective Na+/nucleoside cotransporter mRNA (rCNTl) was detected in samples from each brain region tested by RT-PCR amplification of a 309-bp DNA product. Southern blot and sequence analysis confirmed that this product was derived from rCNTl mRNA. A purine-selective Na+/nucleoside cotransporter mRNA (rCNT2, also termed SPNT) was detected throughout brain by amplifying a 235-bp DNA product, the sequence of which was identical to that published. These experiments demonstrate the presence of both rCNT1 and rCNT2 mRNA in rat brain.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Manitoba</li></region><settlement><li>Winnipeg</li></settlement><orgName><li>Université du Manitoba</li></orgName></list><tree><country name="Canada"><region name="Manitoba"><name sortKey="Anderson, Christopher M" sort="Anderson, Christopher M" uniqKey="Anderson C" first="Christopher M." last="Anderson">Christopher M. Anderson</name></region><name sortKey="Cass, Carol E" sort="Cass, Carol E" uniqKey="Cass C" first="Carol E." last="Cass">Carol E. Cass</name><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E." last="Parkinson">Fiona E. Parkinson</name><name sortKey="Parkinson, Fiona E" sort="Parkinson, Fiona E" uniqKey="Parkinson F" first="Fiona E." last="Parkinson">Fiona E. Parkinson</name><name sortKey="Xiong, Wei" sort="Xiong, Wei" uniqKey="Xiong W" first="Wei" last="Xiong">Wei Xiong</name><name sortKey="Young, James D" sort="Young, James D" uniqKey="Young J" first="James D." last="Young">James D. Young</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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